Analytical validation of two RT-qPCR tests and detection of spring viremia of carp virus (SVCV) in persistently infected koi Cyprinus carpio

Spring viremia of carp virus (SVCV) ia a sprivivirus and member the genus Sprivivirus within family Rhabdoviridae . The is etiological agent spring carp, disease cyprinid species including koi Cyprinus carpio L. notifiable to World Organisation for Animal Health. goal this study was explore hypotheses regarding inter-genogroup (Ia Id) SVCV infection dynamics in juvenile contemporaneously create new reverse-transcription quantitative PCR (RT-qPCR) assays validate their analytical sensitivity, specificity (ASp) repeatability diagnostic detection SVCV. RT-qPCR tests targeting nucleoprotein (Q2N) or glycoprotein (Q1G) nucleotides were pan-specific isolates typed genogroups Ia Id. Q2N test had broader ASp than Q1G because did not detect isolate 20120450 displayed occasional pike fry V76. Neither cross-reacted with other rhabdoviruses, infectious pancreatic necrosis co-localizing herpesvirus 3. Both sensitive observed 50% limits 3 plasmid copies high repeatability. Test analysis immersed showed that could be detected at least 167 d following exposure titer, prevalence, replicative rate persistence correlated significantly virulence. In context, virulence more prevalent, reached higher titers quicker persisted longer periods time relative moderate low isolates.